Composite

Part:BBa_K2622032

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)


His-IgA-Mstx

His-IgA-Mstx has a 6x His-tag fused to translocator domain's (BBa_K2622003) N-terminus, so that it could be displayed and detected in the outer surface of the membrane. On the C-terminus it has a Mstx protein (BBa_K2622006) that improves recombinant membrane protein integration. There is no signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase as it has T7 promoter and terminator.

This composite part has a RFC10 prefix and suffix, though it has incompatible XbaI, EcoRI and PstI sites that must be avoided when cloning.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 207
    Illegal XbaI site found at 39
    Illegal PstI site found at 190
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 207
    Illegal NheI site found at 117
    Illegal PstI site found at 190
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 207
    Illegal BglII site found at 186
    Illegal BamHI site found at 173
    Illegal XhoI site found at 182
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 207
    Illegal XbaI site found at 39
    Illegal PstI site found at 190
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 207
    Illegal XbaI site found at 39
    Illegal PstI site found at 190
    Illegal NgoMIV site found at 1050
    Illegal AgeI site found at 825
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 20


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